C57BL/6-Tg(TcraTcrb)1100Mjb/J

https://www.jax.org/strain/003831

这些小鼠携带了小鼠Tcra-V2和Tcrb-V5基因的转基因插入。这些转基因T细胞受体被设计用来识别在H2Kb（与CD8共受体通过MHC类I分子进行相互作用）背景下的卵清蛋白肽段257-264（OVA257-264）。这导致MHC类I限制性的、卵清蛋白特异性的、CD8+ T细胞（OT-I细胞）的产生。也就是说，这种小鼠的CD8 T细胞主要在MHC I分子的呈递下识别OVA257-264。通过体内过继转移或与携带卵清蛋白转基因的细胞体外共培养，或者通过直接给予卵清蛋白，可以研究免疫反应的动态。这些小鼠通常用于研究CD8+ T细胞对抗原的反应、正向选择以及任何需要定义特异性CD8+ T细胞的研究。像大多数TCR转基因小鼠一样，这些小鼠在某种程度上是免疫缺陷的。

These mice contain transgenic inserts for mouse Tcra-V2 and Tcrb-V5 genes. The transgenic T cell receptor was designed to recognize ovalbumin peptide residues 257-264 (OVA257-264) in the context of H2Kb (CD8 co-receptor interaction with MHC class I). This results in MHC class I-restricted, ovalbumin-specific, CD8+ T cells (OT-I cells). That is, the CD8 T cells of this mouse primarily recognize OVA257-264 when presented by the MHC I molecule. Immune response dynamics can be studied by in vivo adoptive transfer or in vitro co-culture with cells transgenic for ovalbumin or by direct administration of ovalbumin. These mice are often used to study CD8+ T cell response to antigen, positive selection, and in any research requiring CD8+ T cells of defined specificity. Like most TCR transgenics, these mice are somewhat immunodeficient.